ProSci

CD49f Antibody

Product Code:
 
PSI-63-858
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CD49f Antibody in 293 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with CD49f Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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❮❯

Western blot analysis of CD49f Antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD49f Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-858-400ul400ul£626.00
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Further Information

Additional Names:
Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD49f, Integrin alpha-6 heavy chain, Integrin alpha-6 light chain, Processed integrin alpha-6, Alpha6p, ITGA6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The CD49f protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Background References:
  • Kim,T.H., et.al., Mol. Cancer Res.(10), 1605-1612 (2009)
  • Eeles,R.A., et.a.l, Nat. Genet. 41 (10), 1116-1121 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD49f antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1082-1110 amino acids from the C-terminal region of human CD49f.
NCBI Gene ID #:
3655
NCBI Official Name:
Integrin alpha-6
NCBI Official Symbol:
ITGA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
127 kDa
Protein Accession #:
P23229
Protein GI Number:
519668687
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P23229
User NOte:
Optimal dilutions for each application to be determined by the researcher.