Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western Blot at 1:1000 dilution + human blood plasma lysates Lysates/proteins at 20 ug per lane. Western Blot at 1:1000 dilution + human blood plasma lysates Lysates/proteins at 20 ug per lane.](https://www.prosci-inc.com/static-images/55-335_1.jpg)
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![Western blot analysis of HP using rabbit polyclonal HP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene. Western blot analysis of HP using rabbit polyclonal HP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.](https://www.prosci-inc.com/static-images/55-335_2.jpg)
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![](https://www.prosci-inc.com/static-images/55-335_3.jpg)
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![Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](https://www.prosci-inc.com/static-images/55-335_4.jpg)
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![Confocal immunofluorescent analysis of HP Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). Confocal immunofluorescent analysis of HP Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).](https://www.prosci-inc.com/static-images/55-335_5.jpg)
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![Western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the detected the HP protein (arrow). Western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the detected the HP protein (arrow).](https://www.prosci-inc.com/static-images/55-335_6.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
- Documents
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Further Information
Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain, HP
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
This gene encodes a preproprotein, which is processed to
yield both alpha and beta chains, which subsequently combine as a
tetramer to produce haptoglobin. Haptoglobin functions to bind free
plasma hemoglobin, which allows degradative enzymes to gain access
to the hemoglobin, while at the same time preventing loss of iron
through the kidneys and protecting the kidneys from damage by
hemoglobin. Mutations in this gene and/or its regulatory regions
cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also
been linked to diabetic nephropathy, the incidence of coronary
artery disease in type 1 diabetes, Crohn's disease, inflammatory
disease behavior, primary sclerosing cholangitis, susceptibility to
idiopathic Parkinson's disease, and a reduced incidence of
Plasmodium falciparum malaria. A similar duplicated gene is located
next to this gene on chromosome 16. Multiple transcript variants
encoding different isoforms have been found for this gene.
- Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
- Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
- Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
- Savy, M., et al. PLoS ONE(6), E11075 (2010) :
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-322 amino acids from the Central region of human HP.
3240
Haptoglobin
HP
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
P00738
123508
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cell Cycle,Signal Transduction
P00738
Optimal dilutions for each application to be determined by the researcher.