ProSci

CTLA-4 Antibody [1E6]

Product Code:
 
PSI-RF16011
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
CTLA-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.125 and 0.25 μg/mL.
2 / 9
Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
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Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL. <br><br>Red: CTLA4 Antibody [1E6] (RF16011) <br> Blue: DAPI staining
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Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL. <br><br>Red: CTLA4 Antibody [1E6] (RF16011) <br> Blue: DAPI staining
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Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL. <br><br>Red: CTLA4 Antibody [1E6] (RF16011) <br> Blue: DAPI staining
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Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.
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Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.
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Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations.
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A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, RF16013, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.
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Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.125 and 0.25 μg/mL.
Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL. <br><br>Red: CTLA4 Antibody [1E6] (RF16011) <br> Blue: DAPI staining
Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL. <br><br>Red: CTLA4 Antibody [1E6] (RF16011) <br> Blue: DAPI staining
Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL. <br><br>Red: CTLA4 Antibody [1E6] (RF16011) <br> Blue: DAPI staining
Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.
Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.
Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, RF16013, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.

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CodeSizePrice
PSI-RF16011-0.02mg0.02mg£150.00
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PSI-RF16011-0.1mg0.1mg£515.00
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Further Information

Additional Names:
CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3
Application Note:
CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 0.125 - 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
Background References:
  • Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267?70.
  • Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405?13.
  • Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607?9.
  • Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.
Buffer:
CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
NCBI Gene ID #:
1493
NCBI Official Name:
cytotoxic T-lymphocyte associated protein 4
NCBI Official Symbol:
CTLA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 25 kDa

Observed: 32 kDa
Protein Accession #:
NP_005205
Purification:
CTLA-4 Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
P16410
User NOte:
Optimal dilutions for each application to be determined by the researcher.