Further Information
HLA class II histocompatibility antigen, DR beta 5 chain, DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5, HLA-DRB5 {ECO:0000312|EMBL:CAI180791}
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
- Asano, K., et al. Nat. Genet. 41(12):1325-1329(2009)
- Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
- Montano-Loza, A.J., et al. Liver Int. 29(10):1543-1551(2009)
- Lincoln, M.R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(18):7542-7547(2009)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This HLA-DRB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the Central region of human HLA-DRB5.
3127
HLA class II histocompatibility antigen, DR beta 5 chain
HLA-DRB5
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Q30154
74754558
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunology
Q30154
Optimal dilutions for each application to be determined by the researcher.