ProSci

SIKE Antibody

Product Code:
 
PSI-61-907
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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SIKE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
SIKE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-907-400ul400ul£626.00
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Further Information

Additional Names:
Suppressor of IKBKE 1, Suppressor of IKK-epsilon, SIKE1, SIKE
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]).
Background References:
  • Goudreault, M., et al. Mol. Cell Proteomics 8(1):157-171(2009)
  • Huang, J., et al. EMBO J. 24(23):4018-4028(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This SIKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the C-terminal region of human SIKE.
NCBI Gene ID #:
80143
NCBI Official Name:
Suppressor of IKBKE 1
NCBI Official Symbol:
SIKE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q9BRV8
Protein GI Number:
74752290
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q9BRV8
User NOte:
Optimal dilutions for each application to be determined by the researcher.