ProSci

TRAP Antibody

Product Code:
 
PSI-62-315
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of TRAP Antibody in K562 cell line lysates (35ug/lane)
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TRAP Antibody IHC analysis in formalin fixed and paraffin embedded kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of TRAP Antibody in K562 cell line lysates (35ug/lane)
TRAP Antibody IHC analysis in formalin fixed and paraffin embedded kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-315-400ul400ul£626.00
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Further Information

Additional Names:
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Background References:
  • Volmar,C.H., Exp. Cell Res. 315 (13), 2265-2274 (2009)
  • Chai,H., Surgery 146 (1), 5-11 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-207 amino acids from the C-terminal region of human TRAP.
NCBI Gene ID #:
959
NCBI Official Name:
CD40 ligand
NCBI Official Symbol:
CD40LG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P29965
Protein GI Number:
231718
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
P29965
User NOte:
Optimal dilutions for each application to be determined by the researcher.