Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).](https://www.prosci-inc.com/static-images/55-325_1.jpg)
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![Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).](https://www.prosci-inc.com/static-images/55-325_2.jpg)
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![](https://www.prosci-inc.com/static-images/55-325_3.jpg)
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![Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane. Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.](https://www.prosci-inc.com/static-images/55-325_4.jpg)
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![Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane. Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.](https://www.prosci-inc.com/static-images/55-325_5.jpg)
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![Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](https://www.prosci-inc.com/static-images/55-325_6.jpg)
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![Western blot analysis in A549,K562 cell line lysates (35ug/lane). Western blot analysis in A549,K562 cell line lysates (35ug/lane).](https://www.prosci-inc.com/static-images/55-325_7.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:500
For FACS starting dilution is: 1:10~50
CD11b is the integrin alpha M chain. Integrins
are heterodimeric integral membrane proteins composed of an alpha
chain and a beta chain. This I-domain containing alpha integrin
combines with the beta 2 chain (ITGB2) to form a leukocyte-specific
integrin referred to as macrophage receptor 1 ('Mac-1'), or
inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2
integrin is important in the adherence of neutrophils and monocytes
to stimulated endothelium, and also in the phagocytosis of
complement coated particles.
- Gjelstrup, L.C., et al. J. Immunol. 185(7):4154-4168(2010)
- Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
- Warchol, T., et al. DNA Cell Biol. (2010) In press :
- Fan, Y., et al.Eur Acad Dermatol Venereol (2010) In press :
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
3684
Integrin alpha-M
ITGAM
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
127 kDa
P11215
1708572
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunology,Neuroscience,Signal Transduction
P11215
Optimal dilutions for each application to be determined by the researcher.