ProSci

C5AR1 Antibody

Product Code:
 
PSI-55-354
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in A549 cell line lysates (35ug/lane).
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Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in A549 cell line lysates (35ug/lane).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-55-354-400ul400ul£626.00
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Further Information

Additional Names:
C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88, C5AR1, C5AR, C5R1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Background References:
  • Skeie, J.M., et al. Invest. Ophthalmol. Vis. Sci. 51(10):5336-5342(2010)
  • Nishiura, H., et al. Apoptosis 15(8):966-981(2010)
  • Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
  • Marc, M.M., et al. Scand. J. Immunol. 71(5):386-391(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This C5AR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human C5AR1.
NCBI Gene ID #:
728
NCBI Official Name:
C5a anaphylatoxin chemotactic receptor 1
NCBI Official Symbol:
C5AR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
P21730
Protein GI Number:
311033355
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Obesity,Signal Transduction,Growth Factor
Swissprot #:
P21730
User NOte:
Optimal dilutions for each application to be determined by the researcher.