Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western blot analysis in Jurkat cell line lysates (35ug/lane). Western blot analysis in Jurkat cell line lysates (35ug/lane).](https://www.prosci-inc.com/static-images/57-509_1.jpg)
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![ZAP70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ZAP70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.](https://www.prosci-inc.com/static-images/57-509_2.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
This gene encodes an enzyme belonging to the protein
tyrosine kinase family, and it plays a role in T-cell development
and lymphocyte activation. This enzyme, which is phosphorylated on
tyrosine residues upon T-cell antigen receptor (TCR) stimulation,
functions in the initial step of TCR-mediated signal transduction
in combination with the Src family kinases, Lck and Fyn. This
enzyme is also essential for thymocyte development. Mutations in
this gene cause selective T-cell defect, a severe combined
immunodeficiency disease characterized by a selective absence of
CD8-positive T-cells. Two transcript variants that encode different
isoforms have been found for this gene.
- Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010)
- Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) :
- Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010)
- Kotaskova, J., et al.Mol Diagn 12(3):328-334(2010)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse
This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human ZAP70.
7535
Tyrosine-protein kinase ZAP-70
ZAP70
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
P43403
1177044
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunology,Signal Transduction
P43403
Optimal dilutions for each application to be determined by the researcher.