ProSci

BHLHB5 Antibody

Product Code:
 
PSI-61-659
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of BHLHB5 Antibody in HL-60 cell line lysates (35ug/lane)
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BHLHB5 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of BHLHB5 Antibody in HL-60 cell line lysates (35ug/lane)
BHLHB5 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-659-400ul400ul£626.00
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Further Information

Additional Names:
Class E basic helix-loop-helix protein 22, bHLHe22, Class B basic helix-loop-helix protein 5, bHLHb5, Trinucleotide repeat-containing gene 20 protein, BHLHE22, BHLHB5, TNRC20
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
Background References:
  • Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008)
  • McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human BHLHB5.
NCBI Gene ID #:
27319
NCBI Official Name:
Class E basic helix-loop-helix protein 22
NCBI Official Symbol:
BHLHE22
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q8NFJ8
Protein GI Number:
74751284
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q8NFJ8
User NOte:
Optimal dilutions for each application to be determined by the researcher.