ProSci

HNMT Antibody

Product Code:
 
PSI-57-727
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Confocal immunofluorescent analysis of HNMT Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of HNMT Antibody in Hela cell line lysates (35ug/lane). HNMT was detected using the purified polyclonal antibody.
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Confocal immunofluorescent analysis of HNMT Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of HNMT Antibody in Hela cell line lysates (35ug/lane). HNMT was detected using the purified polyclonal antibody.

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PSI-57-727-400ul400ul£626.00
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Further Information

Additional Names:
Histamine N-methyltransferase, HMT, HNMT
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.
Background References:
  • Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
NCBI Gene ID #:
3176
NCBI Official Name:
Histamine N-methyltransferase
NCBI Official Symbol:
HNMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P50135
Protein GI Number:
1708272
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P50135
User NOte:
Optimal dilutions for each application to be determined by the researcher.