ProSci

COL2A1 Antibody

Product Code:
 
PSI-57-548
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-57-548-400ul400ul£626.00
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Further Information

Additional Names:
Collagen alpha-1(II) chain, Alpha-1 type II collagen, Collagen alpha-1(II) chain, Chondrocalcin, COL2A1
Application Note:
For WB starting dilution is: 1:1000
Background:
Alpha-1 chain of type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutant forms of this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Background References:
  • Olavarrieta,L., Clin. Genet. 73 (3), 262-267 (2008)
  • McAlinden,A., Hum. Mutat. 29 (1), 83-90 (2008)
  • Forzano,F., Am. J. Med. Genet.143 (23), 2815-2820 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1209-1237 amino acids from the C-terminal region of human COL2A1.
NCBI Gene ID #:
1280
NCBI Official Name:
Collagen alpha-1(II) chain
NCBI Official Symbol:
COL2A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
142 kDa
Protein Accession #:
P02458
Protein GI Number:
124056489
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P02458
User NOte:
Optimal dilutions for each application to be determined by the researcher.