ProSci

PPP2R2B Antibody

Product Code:
 
PSI-63-541
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PPP2R2B Antibody in Hela cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue with PPP2R2B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PPP2R2B Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue with PPP2R2B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-541-400ul400ul£626.00
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Further Information

Additional Names:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PPP2R2B belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This protein is a beta isoform of the regulatory subunit B55 subfamily.
Background References:
  • Rajkiewicz,M., et.al., Neurol. Neurochir. Pol. 42 (6), 497-504 (2008)
  • Ben-Israel,H., et.al., J. Virol. 82 (19), 9381-9388 (2008)
  • Strack,S., et.al., J. Comp. Neurol. 392 (4), 515-527 (1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus
Immunogen:
This PPP2R2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PPP2R2B.
NCBI Gene ID #:
5521
NCBI Official Name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
NCBI Official Symbol:
PPP2R2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
Q00005
Protein GI Number:
231446
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q00005
User NOte:
Optimal dilutions for each application to be determined by the researcher.