ProSci

Rab5 Antibody

Product Code:
 
PSI-62-565
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of Rab5 Antibody in HL-60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of Rab5 Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-565-400ul400ul£626.00
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Further Information

Additional Names:
Ras-related protein Rab-5A, RAB5A, RAB5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Rab5 is required for the fusion of plasma membranes and early endosomes.
Background References:
  • Farnsworth,C.C., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (25), 11963-11967 (1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Rab5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from the C-terminal region of human Rab5.
NCBI Gene ID #:
5868
NCBI Official Name:
Ras-related protein Rab-5A
NCBI Official Symbol:
RAB5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P20339
Protein GI Number:
1346958
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction,
Swissprot #:
P20339
User NOte:
Optimal dilutions for each application to be determined by the researcher.