Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
- Calero, G., et al., J. Biol. Chem. 278(39):37957-37964 (2003).
- Hofmann, S.L., et al., Curr. Mol. Med. 2(5):423-437 (2002).
- Weimer, J.M., et al., Neuromolecular Med. 1(2):111-124 (2002).
- Lu, J.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 93(19):10046-10050 (1996).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Monkey
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
5538
Palmitoyl-protein thioesterase 1
PPT1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
P50897
1709747
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Obesity,Signal Transduction
P50897
Optimal dilutions for each application to be determined by the researcher.