ProSci

NNMT Antibody

Product Code:
 
PSI-55-106
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of NNMT using rabbit polyclonal NNMT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2).
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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of NNMT using rabbit polyclonal NNMT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2).

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PSI-55-106-400ul400ul£626.00
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Further Information

Additional Names:
Nicotinamide N-methyltransferase, NNMT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is the protein responsible for this enzymatic activity, which uses S-adenosyl methionine as the methyl donor.
Background References:
  • Xu,J., Thyroid 16 (2), 151-160 (2006)
  • Roessler,M., Clin. Cancer Res. 11 (18), 6550-6557 (2005)
  • Souto,J.C., Am. J. Hum. Genet. 76 (6), 925-933 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT.
NCBI Gene ID #:
4837
NCBI Official Name:
Nicotinamide N-methyltransferase
NCBI Official Symbol:
NNMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P40261
Protein GI Number:
730163
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P40261
User NOte:
Optimal dilutions for each application to be determined by the researcher.