ProSci

RHBG Antibody

Product Code:
 
PSI-64-221
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of RHBG Antibody in K562 cell line lysates (35ug/lane)
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RHBG Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of RHBG Antibody in K562 cell line lysates (35ug/lane)
RHBG Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-221-400ul400ul£626.00
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Further Information

Additional Names:
Ammonium transporter Rh type B, Rhesus blood group family type B glycoprotein, Rh family type B glycoprotein, Rh type B glycoprotein, RHBG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
RHBG encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini.
Background References:
  • Han, K.H., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 297 (1), L153-L163 (2009)
  • Brown, A.C., et al. Am. J. Physiol. Renal Physiol. 296 (6), F1279-F1290 (2009)
  • Sohet, F., et al. J. Biol. Chem. 283(39):26557-26567(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RHBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-450 amino acids from the C-terminal region of human RHBG.
NCBI Gene ID #:
57127
NCBI Official Name:
Ammonium transporter Rh type B
NCBI Official Symbol:
RHBG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q9H310
Protein GI Number:
209572666
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9H310
User NOte:
Optimal dilutions for each application to be determined by the researcher.