ProSci

GPT Antibody

Product Code:
 
PSI-62-936
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in HL60 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma wit, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
❮❯

Western blot analysis in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma wit, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-936-400ul400ul£626.00
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Further Information

Additional Names:
Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.
Background References:
  • Yang R.-Z., Blaileanu G.Genomics 79:445-450(2002)
  • Ishiguro M., Takio K.Biochemistry 30:10451-10457(1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-112 amino acids from the N-terminal region of human GPT.
NCBI Gene ID #:
2875
NCBI Official Name:
Alanine aminotransferase 1
NCBI Official Symbol:
GPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
P24298
Protein GI Number:
46577683
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P24298
User NOte:
Optimal dilutions for each application to be determined by the researcher.