ProSci

ACBG2 Antibody

Product Code:
 
PSI-64-149
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 4
Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of ACBG2 Antibody in K562 cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ACBG2 Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-64-149-400ul400ul£626.00
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Further Information

Additional Names:
Long-chain-fatty-acid--CoA ligase ACSBG2, Acyl-CoA synthetase bubblegum family member 2, Bubblegum-related protein, PRTD-NY3, ACSBG2, BGR
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background References:
  • Fraisl, P., et al. Arch. Biochem. Biophys. 451(1):23-33(2006)
  • Pei, Z., et al. J. Biol. Chem. 281(10):6632-6641(2006)
  • Zheng, Y., et al. Asian J. Androl. 7(1):21-32(2005)
  • Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ACBG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-265 amino acids from the Central region of human ACBG2.
NCBI Gene ID #:
81616
NCBI Official Name:
Long-chain-fatty-acid--CoA ligase ACSBG2
NCBI Official Symbol:
ACSBG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74 kDa
Protein Accession #:
Q5FVE4
Protein GI Number:
296434386
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q5FVE4
User NOte:
Optimal dilutions for each application to be determined by the researcher.