ProSci

CYP4B1 Antibody

Product Code:
 
PSI-63-569
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CYP4B1 Antibody in MDA-MB468 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CYP4B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of CYP4B1 Antibody in MDA-MB468 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CYP4B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-569-400ul400ul£626.00
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Further Information

Additional Names:
Cytochrome P450 4B1, CYPIVB1, Cytochrome P450-HP, CYP4B1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
CYP4B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined.
Background References:
  • McKinnon,R.A., et.al., Pharmacogenetics(5), 260-270 (1994)
  • Yokotani,N., et.al., Eur. J. Biochem. 187 (1), 23-29 (1990)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CYP4B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-362 amino acids from the Central region of human CYP4B1.
NCBI Gene ID #:
1580
NCBI Official Name:
Cytochrome P450 4B1
NCBI Official Symbol:
CYP4B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P13584
Protein GI Number:
48429213
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P13584
User NOte:
Optimal dilutions for each application to be determined by the researcher.