Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.](https://www.prosci-inc.com/static-images/63-583_1.jpg)
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![Western blot analysis of NR1I2 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of NR1I2 Antibody in HL-60 cell line lysates (35ug/lane)](https://www.prosci-inc.com/static-images/63-583_2.jpg)
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![](https://www.prosci-inc.com/static-images/63-583_3.jpg)
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![Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](https://www.prosci-inc.com/static-images/63-583_4.jpg)
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![Confocal immunofluorescent analysis of NR1I2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). Confocal immunofluorescent analysis of NR1I2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).](https://www.prosci-inc.com/static-images/63-583_5.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X receptor, Steroid and xenobiotic receptor, SXR, NR1I2, PXR
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. This protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.
- Lehmann,J.M., et.al., J. Clin. Invest. 102 (5), 1016-1023 (1998)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This NR1I2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-127 amino acids from the Central region of human NR1I2.
8856
Nuclear receptor subfamily 1 group I member 2
NR1I2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
O75469
This antibody is purified through a protein A column, followed by peptide affinity purification.
Obesity,Signal Transduction
O75469
Optimal dilutions for each application to be determined by the researcher.