ProSci

ADD3 Antibody

Product Code:
 
PSI-57-571
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

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PSI-57-571-400ul400ul£626.00
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Further Information

Additional Names:
Gamma-adducin, Adducin-like protein 70, ADD3, ADDL
Application Note:
For WB starting dilution is: 1:1000
Background:
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Garcia-Barcelo, M.M., et al. Hum. Mol. Genet. 19(14):2917-2925(2010)
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ADD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 552-580 amino acids from the C-terminal region of human ADD3.
NCBI Gene ID #:
120
NCBI Official Name:
Gamma-adducin
NCBI Official Symbol:
ADD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
Q9UEY8
Protein GI Number:
12643881
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9UEY8
User NOte:
Optimal dilutions for each application to be determined by the researcher.