ProSci

P4HA1 Antibody

Product Code:
 
PSI-58-934
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Ramos cell line lysates (35ug/lane).
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Western blot analysis in Ramos cell line lysates (35ug/lane).

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PSI-58-934-400ul400ul£626.00
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Further Information

Additional Names:
Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Gorres, K.L., et al. PLoS ONE(11), E7635 (2009) :
  • Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
  • Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1.
NCBI Gene ID #:
5033
NCBI Official Name:
Prolyl 4-hydroxylase subunit alpha-1
NCBI Official Symbol:
P4HA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P13674
Protein GI Number:
2507090
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P13674
User NOte:
Optimal dilutions for each application to be determined by the researcher.