ProSci

RAB27B Antibody

Product Code:
 
PSI-61-743
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of RAB27B Antibody in NCI-H460 cell line lysates (35ug/lane)
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of RAB27B Antibody in NCI-H460 cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-743-400ul400ul£626.00
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Further Information

Additional Names:
Ras-related protein Rab-27B, C25KG, RAB27B
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
RAB27B may be involved in targeting uroplakins to urothelial apical membranes.
Background References:
  • Hayasaka, S., et al. Asian J. Androl. 10(4):561-568(2008)
  • Shirakawa, R., et al. J. Biol. Chem. 279(11):10730-10737(2004)
  • Tiwari, S., et al. Blood 102(12):3970-3979(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RAB27B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the C-terminal region of human RAB27B.
NCBI Gene ID #:
5874
NCBI Official Name:
Ras-related protein Rab-27B
NCBI Official Symbol:
RAB27B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
O00194
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O00194
User NOte:
Optimal dilutions for each application to be determined by the researcher.