Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
The protein encoded by this gene is one subunit of a
trimeric complex, forming a highly conserved transcription factor
that binds with high specificity to CCAAT motifs in the promoter
regions in a variety of genes. This gene product, subunit B, forms
a tight dimer with the C subunit, a prerequisite for subunit A
association. The resulting trimer binds to DNA with high
specificity and affinity. Subunits B and C each contain a
histone-like motif. Observation of the histone nature of these
subunits is supported by two types of evidence; protein sequence
alignments and experiments with mutants.
- Stros, M., et al. Nucleic Acids Res. 37(7):2070-2086(2009)
- Kang, J.H., et al. J. Cell. Biochem. 104(4):1192-1203(2008)
- Raju, C.S., et al. Mol. Biol. Cell 19(7):3008-3019(2008)
- Di Agostino, S., et al. Cancer Cell 10(3):191-202(2006)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.
4801
Nuclear transcription factor Y subunit beta
NFYB
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
P25208
399193
This antibody is purified through a protein A column, followed by peptide affinity purification.
Signal Transduction
P25208
Optimal dilutions for each application to be determined by the researcher.