ProSci

MPP3 Antibody

Product Code:
 
PSI-56-830
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 5
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
3 / 5
4 / 5
Confocal immunofluorescent analysis of MPP3 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
5 / 5
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Confocal immunofluorescent analysis of MPP3 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-830-400ul400ul£626.00
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Further Information

Additional Names:
MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3, MPP3, DLG3
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.
Background References:
  • Qin, L., et al. Zhonghua Gan Zang Bing Za Zhi 15(7):509-512(2007)
  • Kantardzhieva, A., et al. FEBS J. 273(6):1152-1165(2006)
  • Stelzl, U., et al. Cell 122(6):957-968(2005)
  • Fukuhara, H., et al. Oncogene 22(40):6160-6165(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3.
NCBI Gene ID #:
4356
NCBI Official Name:
MAGUK p55 subfamily member 3
NCBI Official Symbol:
MPP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q13368
Protein GI Number:
150421601
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q13368
User NOte:
Optimal dilutions for each application to be determined by the researcher.