ProSci

ARHGEF3 Antibody

Product Code:
 
PSI-62-060
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
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ARHGEF3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
ARHGEF3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-060-400ul400ul£626.00
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Further Information

Additional Names:
Rho guanine nucleotide exchange factor 3, Exchange factor found in platelets and leukemic and neuronal tissues, XPLN, ARHGEF3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Arthur, W.T., et al. J. Biol. Chem. 277(45):42964-42972(2002)
  • Harrington, A.W., et al. J. Neurosci. 22(1):156-166(2002)
  • Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
  • Thiesen, S., et al. Biochem. Biophys. Res. Commun. 273(1):364-369(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Monkey, Mouse
Immunogen:
This ARHGEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human ARHGEF3.
NCBI Gene ID #:
50650
NCBI Official Name:
Rho guanine nucleotide exchange factor 3
NCBI Official Symbol:
ARHGEF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
Q9NR81
Protein GI Number:
52782760
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9NR81
User NOte:
Optimal dilutions for each application to be determined by the researcher.