ProSci

DUSP8 Antibody

Product Code:
 
PSI-63-478
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of anti-DUSP8 Pabin Hela cell line lysate (35ug/lane).
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
❮❯

Western blot analysis of anti-DUSP8 Pabin Hela cell line lysate (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-63-478-400ul400ul£626.00
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Further Information

Additional Names:
Dual specificity protein phosphatase 8, Dual specificity protein phosphatase hVH-5, DUSP8, C11orf81, VH5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.
Background References:
  • Berger, I.R., et al., Cancer Genet. Cytogenet. 159(2):155-159 (2005).
  • Hink, R.L., et al., Genomics 8(3):305-312 (2003).
  • Nesbit, M.A., et al., Genomics 42(2):284-294 (1997).
  • Martell, K.J., et al., J. Neurochem. 65(4):1823-1833 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DUSP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-487 amino acids from the C-terminal region of human DUSP8.
NCBI Gene ID #:
1850
NCBI Official Name:
Dual specificity protein phosphatase 8
NCBI Official Symbol:
DUSP8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q13202
Protein GI Number:
223590200
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q13202
User NOte:
Optimal dilutions for each application to be determined by the researcher.