ProSci

RASGRF1 Antibody

Product Code:
 
PSI-63-012
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of RASGRF1 antibody (S929) in MDA-MB231 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of RASGRF1 antibody (S929) in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-012-400ul400ul£626.00
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Further Information

Additional Names:
Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, Guanine nucleotide-releasing protein, GNRP, Ras-specific nucleotide exchange factor CDC25, RASGRF1, CDC25, GNRP, GRF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
Background References:
  • Iguchi,T., Int. J. Oncol. 31 (2), 285-291 (2007)
  • Holmes,R., Mol. Cell. Biol. 26 (24), 9564-9570 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RASGRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 907-936 amino acids from human RASGRF1.
NCBI Gene ID #:
5923
NCBI Official Name:
Ras-specific guanine nucleotide-releasing factor 1
NCBI Official Symbol:
RASGRF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
145 kDa
Protein Accession #:
Q13972
Protein GI Number:
13124259
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q13972
User NOte:
Optimal dilutions for each application to be determined by the researcher.