Further Information
Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, PSF-1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, TAP1, ABCB2, PSF1, RING4, Y3
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
TAP is an integral transmembrane protein involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. TAP is inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. It is inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP and prevents the conformational rearrangement of TAP induced by peptide binding. TAP is also inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP is down-regulated by human Epstein-barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. TAP1 and TAP2 form a heterodimer of TAP1 and TAP2, and the peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. TAP, inducible by interferon gamma, belongs to the ABC transporter family, MDR subfamily.
- Lajoie, J., et al., Hum. Immunol. 64(8):823-829 (2003).
- Gaudet, R., et al., EMBO J. 20(17):4964-4972 (2001).
- Tang, J., et al., Hum. Immunol. 62(3):256-268 (2001).
- Hewitt, E.W., et al., EMBO J. 20(3):387-396 (2001).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This TAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 765-794 amino acids from the C-terminal region of human TAP1.
6890
Antigen peptide transporter 1
TAP1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Q03518
215273957
This antibody is purified through a protein A column, followed by peptide affinity purification.
Signal Transduction
Q03518
Optimal dilutions for each application to be determined by the researcher.