ProSci

ACTN3 Antibody

Product Code:
 
PSI-56-350
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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ACTN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
ACTN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-350-400ul400ul£626.00
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Further Information

Additional Names:
Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments
Background References:
  • Rodriguez-Romo, G., et al. Eur. J. Appl. Physiol. 110(6):1099-1106(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Berman, Y., et al. Physiology (Bethesda) 25(4):250-259(2010)
  • Ruiz, J.R., et al. ScandMed Sci Sports (2010) In press :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ACTN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-437 amino acids from the Central region of human ACTN3.
NCBI Gene ID #:
89
NCBI Official Name:
Alpha-actinin-3
NCBI Official Symbol:
ACTN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
103 kDa
Protein Accession #:
Q08043
Protein GI Number:
215273967
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q08043
User NOte:
Optimal dilutions for each application to be determined by the researcher.