ProSci

SGEF Antibody

Product Code:
 
PSI-56-989
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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SGEF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
SGEF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-989-400ul400ul£626.00
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Further Information

Additional Names:
Rho guanine nucleotide exchange factor 26, SH3 domain-containing guanine exchange factor, ARHGEF26, SGEF
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
SGEF activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry.
Background References:
  • Yamada, S., et al. Oncogene 23(35):5901-5911(2004)
  • Ellerbroek, S.M., et al. Mol. Biol. Cell 15(7):3309-3319(2004)
  • Qi, H., et al. Endocrinology 144(5):1742-1752(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-406 amino acids from the Central region of human SGEF.
NCBI Gene ID #:
26084
NCBI Official Name:
Rho guanine nucleotide exchange factor 26
NCBI Official Symbol:
ARHGEF26
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q96DR7
Protein GI Number:
317373555
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q96DR7
User NOte:
Optimal dilutions for each application to be determined by the researcher.