ProSci

PIGM Antibody

Product Code:
 
PSI-61-649
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PIGM Antibody in T47D cell line lysates (35ug/lane)
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PIGM Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of PIGM Antibody in T47D cell line lysates (35ug/lane)
PIGM Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-649-400ul400ul£626.00
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Further Information

Additional Names:
GPI mannosyltransferase 1, 241-, GPI mannosyltransferase I, GPI-MT-I, Phosphatidylinositol-glycan biosynthesis class M protein, PIG-M, PIGM
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PIGM encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. PIGM encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum.
Background References:
  • Almeida, A.M., et al. Nat. Med. 12(7):846-851(2006) Maeda, Y., et al. EMBO J. 20 (1-2), 250-261 (2001) Kinoshita, T., et al. Curr Opin Chem Biol 4(6):632-638(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PIGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human PIGM.
NCBI Gene ID #:
93183
NCBI Official Name:
GPI mannosyltransferase 1
NCBI Official Symbol:
PIGM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
Q9H3S5
Protein GI Number:
74752622
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H3S5
User NOte:
Optimal dilutions for each application to be determined by the researcher.