ProSci

HRIHFB2025 Antibody

Product Code:
 
PSI-63-805
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of HRIHFB2025 Antibody in MDA-MB231 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of HRIHFB2025 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-805-400ul400ul£626.00
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Further Information

Additional Names:
Protein chibby homolog 1, ARPP-binding protein, Cytosolic leucine-rich protein, PIGEA-14, PKD2 interactor, Golgi and endoplasmic reticulum-associated 1, CBY1, ARB1, C22orf2, CBY, PGEA1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Inhibits the Wnt/Wingless pathway by binding to beta catenin and inhibiting beta catenin mediated transcriptional activation by competing with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin 2/PKD2 and possibly of other intracellular proteins.
Background References:
  • Gauci S., et.al., Anal. Chem. 81:4493-4501(2009).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HRIHFB2025 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the C-terminal region of human HRIHFB2025.
NCBI Gene ID #:
25776
NCBI Official Name:
Protein chibby homolog 1
NCBI Official Symbol:
CBY1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
Q9Y3M2
Protein GI Number:
20454882
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9Y3M2
User NOte:
Optimal dilutions for each application to be determined by the researcher.