ProSci

PDIA6 Antibody

Product Code:
 
PSI-62-386
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis of in NCI-H460, MCF-7, A2058 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
❮❯

Western blot analysis of in NCI-H460, MCF-7, A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-386-400ul400ul£626.00
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Further Information

Additional Names:
Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
Background References:
  • Hayano,T.,Gene 164 (2), 377-378 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human PDIA6.
NCBI Gene ID #:
10130
NCBI Official Name:
Protein disulfide-isomerase A6
NCBI Official Symbol:
PDIA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q15084
Protein GI Number:
2501205
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q15084
User NOte:
Optimal dilutions for each application to be determined by the researcher.