ProSci

PHTNS Antibody

Product Code:
 
PSI-64-226
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PHTNS Antibody in Ramos cell line lysates (35ug/lane)
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PHTNS Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of PHTNS Antibody in Ramos cell line lysates (35ug/lane)
PHTNS Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-226-400ul400ul£626.00
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Further Information

Additional Names:
Phostensin, Protein phosphatase 1 F-actin cytoskeleton-targeting subunit, Protein phosphatase 1 regulatory subunit 18, PPP1R18, HKMT1098, KIAA1949
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PHTNS interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin cytoskeleton。
Background References:
  • Lai, N.S., et al. Biochem. Biophys. Res. Commun. 387(4):676-681(2009)
  • Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009)
  • Kao, S.C., et al. Biochem. Biophys. Res. Commun. 356(3):594-598(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PHTNS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the N-terminal region of human PHTNS.
NCBI Gene ID #:
170954
NCBI Official Name:
Phostensin
NCBI Official Symbol:
PPP1R18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q6NYC8
Protein GI Number:
68052323
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q6NYC8
User NOte:
Optimal dilutions for each application to be determined by the researcher.