ProSci

RASIP1 Antibody

Product Code:
 
PSI-62-513
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-513-400ul400ul£626.00
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Further Information

Additional Names:
Ras-interacting protein 1, Rain, RASIP1
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
RASIP1 may act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.
Background References:
  • Mitin,N.Y.,et.al., J. Biol. Chem. 279 (21), 22353-22361 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RASIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-678 amino acids from the Central region of human RASIP1.
NCBI Gene ID #:
54922
NCBI Official Name:
Ras-interacting protein 1
NCBI Official Symbol:
RASIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
103 kDa
Protein Accession #:
Q5U651
Protein GI Number:
74736209
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q5U651
User NOte:
Optimal dilutions for each application to be determined by the researcher.