ProSci

BGN Antibody

Product Code:
 
PSI-62-440
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of BGN using rabbit polyclonal BGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.
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Western blot analysis of BGN Antibody in Sk-Br-3 cell line lysates (35ug/lane)
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Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of BGN using rabbit polyclonal BGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.
Western blot analysis of BGN Antibody in Sk-Br-3 cell line lysates (35ug/lane)
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-440-400ul400ul£626.00
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Further Information

Additional Names:
Biglycan, Bone/cartilage proteoglycan I, PG-S1, BGN, SLRR1A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
BGN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival.
Background References:
  • Sardo,M.A., Clin. Chim. Acta 406 (1-2), 89-93 (2009)
  • Hwang,J.Y., Am. J. Pathol. 173 (6), 1919-1928 (2008)
  • Traupe,H., Genomics 13 (2), 481-483 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This BGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the Central region of human BGN.
NCBI Gene ID #:
633
NCBI Official Name:
Biglycan
NCBI Official Symbol:
BGN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P21810
Protein GI Number:
266762
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P21810
User NOte:
Optimal dilutions for each application to be determined by the researcher.