ProSci

SYNCI Antibody

Product Code:
 
PSI-55-432
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).
2 / 3
SYNCI Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYNCI Antibody (N-term)for immunohistochemistry.
3 / 3
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Western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).
SYNCI Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYNCI Antibody (N-term)for immunohistochemistry.

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PSI-55-432-400ul400ul£626.00
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Further Information

Additional Names:
Syncoilin, Syncoilin intermediate filament 1, Syncoilin-1, SYNC, SYNC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the intermediate filament family which contains an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. The protein is highly expressed in skeletal and cardiac muscle. The protein links the dystrophin associated protein complex (DAPC) to desmin filaments in muscle and may have a structural role in striated muscle. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Wakayama, Y., et al. Int. J. Neurosci. 120(2):144-149(2010)
  • Jordanova, A., et al. Nat. Genet. 38(2):197-202(2006)
  • Brown, S.C., et al. Muscle Nerve 32(6):715-725(2005)
  • Poon, E., et al. J. Biol. Chem. 277(5):3433-3439(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SYNCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the N-terminal region of human SYNCI.
NCBI Gene ID #:
81493
NCBI Official Name:
Syncoilin
NCBI Official Symbol:
SYNC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q9H7C4
Protein GI Number:
300669677
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H7C4
User NOte:
Optimal dilutions for each application to be determined by the researcher.