ProSci

COL21A1 Antibody

Product Code:
 
PSI-56-246
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HepG2 cell line lysates (35ug/lane).
2 / 3
COL21A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in HepG2 cell line lysates (35ug/lane).
COL21A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-246-400ul400ul£626.00
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Further Information

Additional Names:
Collagen alpha-1(XXI) chain, COL21A1, COL1AL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
This gene encodes the alpha chain of type XXI collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XXI collagen is localized to tissues containing type I collagen so, like other members of this collagen family, it may serve to maintain the integrity of the extracellular matrix. An alternatively spliced transcript variant has been described, but its full-length nature has yet to be determined.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Chou, M.Y., et al. Genomics 79(3):395-401(2002)
  • Tuckwell, D. Matrix Biol. 21(1):63-66(2002)
  • Fitzgerald, J., et al. FEBS Lett. 505(2):275-280(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL21A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the N-terminal region of human COL21A1.
NCBI Gene ID #:
81578
NCBI Official Name:
Collagen alpha-1(XXI) chain
NCBI Official Symbol:
COL21A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
99 kDa
Protein Accession #:
Q96P44
Protein GI Number:
74752071
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q96P44
User NOte:
Optimal dilutions for each application to be determined by the researcher.