ProSci

ADRB2 Antibody

Product Code:
 
PSI-62-774
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 5
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western blot analysis of lysate from HepG2 cell line, using ADRB2 Antibody (S364) at 1:1000.
3 / 5
4 / 5
Formalin-fixed and paraffin-embedded human brain reacted with ADRB2 Antibody (S364), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
5 / 5
Flow cytometric analysis of NCI-H292 cells using ADRB2 Antibody (S364)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from HepG2 cell line, using ADRB2 Antibody (S364) at 1:1000.
Formalin-fixed and paraffin-embedded human brain reacted with ADRB2 Antibody (S364), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using ADRB2 Antibody (S364)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-774-400ul400ul£626.00
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Further Information

Additional Names:
Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor, ADRB2, ADRB2R, B2AR
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ADRB2, beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This protein is intronless.
Background References:
  • Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. U.S.A. 84:46-50(1987)
  • Emorine L.J., Marullo S.Proc. Natl. Acad. Sci. U.S.A. 84:6995-6999(1987)
  • Kobilka B.K., Frielle T.J. Biol. Chem. 262:7321-7327(1987)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ADRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-373 amino acids from human ADRB2.
NCBI Gene ID #:
154
NCBI Official Name:
Beta-2 adrenergic receptor
NCBI Official Symbol:
ADRB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
P07550
Protein GI Number:
296439450
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction,Growth Factor
Swissprot #:
P07550
User NOte:
Optimal dilutions for each application to be determined by the researcher.