ProSci

HIST2H3A Antibody

Product Code:
 
PSI-60-649
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).

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PSI-60-649-400ul400ul£626.00
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Further Information

Additional Names:
Histone H32, Histone H3/m, Histone H3/o, HIST2H3A
Application Note:
For WB starting dilution is: 1:1000
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].
Background References:
  • Neumann, H., et al. Mol. Cell 36(1):153-163(2009)
  • Hurd, P.J., et al. J. Biol. Chem. 284(24):16575-16583(2009)
  • Yuan, J., et al. Cell Cycle 8(11):1747-1753(2009)
  • Chang, Q., et al. J. Hepatol. 50(2):323-333(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Chicken, Zebrafish, Xenopus, C. Elegans, Drosophila, Pig, Rabbit
Immunogen:
This HIST2H3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the C-terminal region of human HIST2H3A.
NCBI Gene ID #:
126961, 333932, 653604
NCBI Official Name:
Histone H3.2
NCBI Official Symbol:
HIST2H3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
Q71DI3
Protein GI Number:
74758899
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q71DI3
User NOte:
Optimal dilutions for each application to be determined by the researcher.