ProSci

EHMT1 Antibody

Product Code:
 
PSI-55-076
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of EHMT1 using rabbit polyclonal EHMT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2).
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Western blot analysis of EHMT1 using rabbit polyclonal EHMT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2).

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PSI-55-076-400ul400ul£626.00
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Further Information

Additional Names:
Histone-lysine N-methyltransferase EHMT1, 211-, Euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, G9a-like protein 1, GLP, GLP1, Histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, Lysine N-methyltransferase 1D, EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D
Application Note:
For WB starting dilution is: 1:1000
Background:
EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
Background References:
  • Ogawa H.,Science 296:1132-1136(2002).
  • Ota T.,Nat. Genet. 36:40-45(2004).
  • Nagase T.,DNA Res. 8:85-95(2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-233 amino acids from the N-terminal region of human EHMT1.
NCBI Gene ID #:
79813
NCBI Official Name:
Histone-lysine N-methyltransferase EHMT1
NCBI Official Symbol:
EHMT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
141 kDa
Protein Accession #:
Q9H9B1
Protein GI Number:
325511404
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9H9B1
User NOte:
Optimal dilutions for each application to be determined by the researcher.