ProSci

H1FX Antibody

Product Code:
 
PSI-57-230
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-57-230-400ul400ul£626.00
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Further Information

Additional Names:
Histone H1x, H1FX
Application Note:
For WB starting dilution is: 1:1000
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family.
Background References:
  • Warneboldt, J., et al. BMC Cancer 8, 388 (2008) :
  • Stoldt, S., et al. Biol. Cell 99(10):541-552(2007)
  • Takata, H., et al. FEBS Lett. 581(20):3783-3788(2007)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This H1FX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the C-terminal region of human H1FX.
NCBI Gene ID #:
8971
NCBI Official Name:
Histone H1x
NCBI Official Symbol:
H1FX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
Q92522
Protein GI Number:
6016184
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q92522
User NOte:
Optimal dilutions for each application to be determined by the researcher.