ProSci

PRMT6 Antibody

Product Code:
 
PSI-55-036
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: LNCaP whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: LNCaP whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-55-036-400ul400ul£626.00
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Further Information

Additional Names:
Protein arginine N-methyltransferase 6, 211-, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, PRMT6, HRMT1L6
Application Note:
For WB starting dilution is: 1:2000
Background:
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Background References:
  • Frankel A., et al. J. Biol. Chem. 277:3537-3543(2002). Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003). Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001). Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999). Gilbreth, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(25):14781-14786 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PRMT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from the N-terminal region of human PRMT6.
NCBI Gene ID #:
55170
NCBI Official Name:
Protein arginine N-methyltransferase 6
NCBI Official Symbol:
PRMT6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q96LA8
Protein GI Number:
20137409
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96LA8
User NOte:
Optimal dilutions for each application to be determined by the researcher.