ProSci

RSPO4 Antibody

Product Code:
 
PSI-62-016
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in Jurkat cell line lysates (15ug/lane).
2 / 3
RSPO4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
❮❯

Western blot analysis in Jurkat cell line lysates (15ug/lane).
RSPO4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-62-016-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
R-spondin-4, Roof plate-specific spondin-4, hRspo4, RSPO4, C20orf182
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
RSPO4 is a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
Background References:
  • Bergmann, C., et al. Am. J. Hum. Genet. 79(6):1105-1109(2006)
  • Blaydon, D.C., et al. Nat. Genet. 38(11):1245-1247(2006)
  • Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RSPO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human RSPO4.
NCBI Gene ID #:
343637
NCBI Official Name:
R-spondin-4
NCBI Official Symbol:
RSPO4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
Q2I0M5
Protein GI Number:
97189858
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q2I0M5
User NOte:
Optimal dilutions for each application to be determined by the researcher.