ProSci

ZO1 Antibody

Product Code:
 
PSI-61-661
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ZO1 Antibody in K562 cell line lysates (35ug/lane)
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ZO1 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of ZO1 Antibody in K562 cell line lysates (35ug/lane)
ZO1 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-661-400ul400ul£626.00
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Further Information

Additional Names:
Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions.
Background References:
  • Ohira, M., et al. Int. J. Mol. Med. 24(6):829-835(2009)
  • Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009)
  • Meerschaert, K., et al. Cell. Mol. Life Sci. 66(24):3951-3966(2009)
  • Kirschner, N., et al. Am. J. Pathol. 175(3):1095-1106(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1570-1600 amino acids from the C-terminal region of human ZO1.
NCBI Gene ID #:
7082
NCBI Official Name:
Tight junction protein ZO-1
NCBI Official Symbol:
TJP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
195 kDa
Protein Accession #:
Q07157
Protein GI Number:
85700443
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q07157
User NOte:
Optimal dilutions for each application to be determined by the researcher.