ProSci

GTF2I Antibody

Product Code:
 
PSI-62-518
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Fluorescent image of Hela cells stained with GTF2I Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 6
Western blot analysis of lysates from A431, Hela cell line (from left to right), using GTF2I Antibody at 1:1000 at each lane.
3 / 6
4 / 6
Fluorescent image of A549 cell stained with GTF2I Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).GTF2I immunoreactivity is localized to Nucleus significantly.
5 / 6
Formalin-fixed and paraffin-embedded human brain tissue reacted with GTF2I Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
6 / 6
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Fluorescent image of Hela cells stained with GTF2I Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from A431, Hela cell line (from left to right), using GTF2I Antibody at 1:1000 at each lane.
Fluorescent image of A549 cell stained with GTF2I Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).GTF2I immunoreactivity is localized to Nucleus significantly.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GTF2I Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-518-400ul400ul£626.00
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Further Information

Additional Names:
General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, BAP-135, BTK-associated protein 135, SRF-Phox1-interacting protein, SPIN, Williams-Beuren syndrome chromosomal region 6 protein, GTF2I, BAP135, WBSCR6
Application Note:
For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
GTF2I is a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23.
Background References:
  • Roy,A.L.,et.al., EMBO J. 16 (23), 7091-7104 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I.
NCBI Gene ID #:
2969
NCBI Official Name:
General transcription factor II-I
NCBI Official Symbol:
GTF2I
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
112 kDa
Protein Accession #:
P78347
Protein GI Number:
17865459
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P78347
User NOte:
Optimal dilutions for each application to be determined by the researcher.