ProSci

MED8 Antibody

Product Code:
 
PSI-56-565
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Western blot analysis in HL-60 cell line lysates (35ug/lane).

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PSI-56-565-400ul400ul£626.00
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Further Information

Additional Names:
Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.
Background References:
  • Leonard, V.H., et al. J. Virol. 80(19):9667-9675(2006)
  • Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
  • Sato, S., et al. Mol. Cell 14(5):685-691(2004)
  • Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse
Immunogen:
This MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-122 amino acids from the Central region of human MED8.
NCBI Gene ID #:
112950
NCBI Official Name:
Mediator of RNA polymerase II transcription subunit 8
NCBI Official Symbol:
MED8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q96G25
Protein GI Number:
31076772
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96G25
User NOte:
Optimal dilutions for each application to be determined by the researcher.