ProSci

EIF5AL1 Antibody

Product Code:
 
PSI-56-158
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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EIF5AL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).
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EIF5AL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).

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PSI-56-158-400ul400ul£626.00
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Further Information

Additional Names:
Eukaryotic translation initiation factor 5A-1-like, eIF-5A-1-like, eIF-5A1-like, Eukaryotic initiation factor 5A isoform 1-like, EIF5AL1
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
EIF5AL1is a mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
Background References:
  • Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
  • Clement, P.M., et al. FEBS J. 273(6):1102-1114(2006)
  • Facchiano, A.M., et al. Protein Eng. 14(11):881-890(2001)
  • Lipowsky, G., et al. EMBO J. 19(16):4362-4371(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EIF5AL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the C-terminal region of human EIF5AL1.
NCBI Gene ID #:
143244
NCBI Official Name:
Eukaryotic translation initiation factor 5A-1-like
NCBI Official Symbol:
EIF5AL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
Q6IS14
Protein GI Number:
190359775
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q6IS14
User NOte:
Optimal dilutions for each application to be determined by the researcher.